2025 has been the biggest year for Artificial Intelligence (AI). It was a year to remember for most US AI startups, and $100M become a common standard in the industry. Top funded AI startups USA have nearly changed every industry we work in.
While fewer companies raised less than $1 billion, many companies were able to raise $100M or more. And with those numbers, investors are betting high in the AI industry.
Following the previous year, there were a total of 49 startups that raised funding rounds worth $100 million or more. In this article, we have rounded up the top US-based AI startups raised $100M or more, covering healthcare, legal, enterprise search, robotics, infrastructure, and other sectors. AI, robotics, autonomous intelligence, foundation model labs, infrastructure, and compute are the major breakthroughs that have driven the massive funding in 2025.
Top 10 US AI Startups That Raised $100M Or More
Funding for the US AI startup ecosystem exploded in 2025. Below is a list of the top-funded AI startups in the USA that raised $100M or more, underscoring the need for strategic capital to secure global AI leadership. Faster, cheaper technology, reduced enterprise costs, safe automation, and clear commercial use cases are among the factors that make these AI startups investor magnets.
1. OpenAI
OpenAI is one of the AI unicorn startups 2025 that was able to raise a record-breaking funding amount of $40 billion round. It is the most popular AI research company behind the advanced LLMs. With the closing of the round on 31st March, the funds raised valued the startup at $300 billion. Through GPT models and multimodal systems, the startups advance generative AI.
2. EliseAI
In artificial intelligence funding rounds 2025, EliseAI raised $250 million in a Series E round. It is one of the top AI startups in the US, providing advanced conversational AI agents. The primary aim of this startup is to automate the housing and healthcare workflows. The funds raised by EliseAI valued it at $2.2 billion.
3. Decart
In AI infrastructure startups funding, Decart raised $100 million in a Series B round, valuing the company at a $3.1 billion. It is a fast-growing AI startup that leads the large language model startups USA list. Decart develops next-gen foundations models for developers and enterprises in San Francisco. With real-time generative AI, it creates interactive experiences.
4. Fal
Fal is one of the most innovative generative AI companies in the US. The company raised $125 million Series C round. On July 31st, the company announced the round, which valued Fal at $1.5 billion. It is a generative media AI infrastructure startup that provides a platform for running video, images, and audio at scale.
5. Ambience Healthcare
Ambience Healthcare is a venture capital AI startups US that raised a $243 million Series C round. The same round was led by Oak HC/FT and Andreessen Horowitz. It is an emerging healthcare AI development startup with the primary goal of automating clinical workflows. By using medical LLMs, the company improves doctors’ efficiency and reduces clinician burden.
6. Glean
In enterprise AI startups funding, Glean raised $150 million in a Series F round. The round was led by Wellington Management, Sequoia, and Lightspeed Venture Partners. On June 10, the company announced and it was valued at $7.25 billion. It is an AI enterprise search and productivity startup that automates workflows and helps employees find knowledge.
7. Hippocratic AI
Hippocratic AI is one of the AI agent startups US that mainly focuses on building safety-first generative AI agents for healthcare sector. It assists non-diagnostic clinical and administrative tasks. On January 9th, the company raised $141 million Series B round and was valued at $1.6 billion. This round was led by Kleiner Perkins, Andreessen Horowitz, and Nvidia.
8. Harmonic
Harmonic is a US-based AI startup that builds mathematical reasoning engines. The company’s models solve unique and complex mathematical tasks. Harmonic raised a $100 million Series B round, which was announced on July 10, valuing the company at $875 million. The company is developing Mathematical Superintelligence (MSI) to ensure accuracy in critical applications.
9. LMArena
Next on the list of US AI startups funding 2025, is LMArena, a top AI benchmarking startup. It specializes in building community-driven platforms for evaluation and leaderboards. It provides real-time insights through crowdsourced feedback. LMArena raised a $100 million seed round, valuing this autonomous AI startups funding at $600 million.
10. Reka AI
Reka AI is one of the AI inference startups USA, that raised $110 million. The same round was led by bSnowflake and Nvidia. The company was valued at $1 billion following the announcement of the Series B round on July 22nd. It is one of the best AI research lab that builds large multimodal, multilingual models that understands video, text, and images.
Final Thoughts!
There you have it, top-funded AI startups USA that raised $100M or more. This is just the beginning, as we enter a new era in 2026, where AI chip startups’ funding is changing the global tech landscape. These startups are building AI infrastructure, tools, and model labs that will define the coming tomorrow of every industry. Hire a dedicated AI developer to integrate AI into your business. The widespread adoption of Generative AI across every industry and the increase in infrastructure costs are the two main reasons AI startup funding has reached this level.
